Journal: Nature Communications

Article Title: Aggregation-induced emission luminogens for image-guided surgery in non-human primates

doi: 10.1038/s41467-021-26417-2

Figure Lengend Snippet: a Representative process of folic-AIEgen guided LN dissection in nude mice ( n = 3). b Images of the lymphatic vessels (red arrows) and SLNs (red arrowheads) under white light and a handheld UV lamp. i-Before injection, ii-30 seconds after injection of folic-AIEgen (25 µL, 40 µg/mL) into the front footpad of mice, iii-Dissection of the LN. c Photographs of draining LN and contralateral LN obtained from IVIS system and digital camera. d Quantitative analysis of the fluorescence intensity of draining LNs and contralateral LN. Data are means ± SD, n = 3, Student’s two-tailed t test, *** P < 0.001. e Representative process of folic-AIEgen guided SLNs dissection from the armpit of rabbit ( n = 3). f Images of the lymphatic vessels (red arrows) and SLNs (red arrowheads) under white light and a handheld UV lamp. i and ii-Before injection, iii-3 min after injection of folic-AIEgen (500 µL, 40 µg/mL) around the second papilla of rabbit, iv-Separation along the lymph vessel, v and vi- Dissection of the SLN. g Photographs of draining LN and contralateral LN obtained from the digital camera under white and UV light. h Histological analysis of the draining LN and contralateral LN. Representative bright field and photoluminescence images at 488 nm excitation of frozen sections. Histological changes were evaluated by H&E staining. Scale bars = 100 μm. The experiment was repeated at least three times.

Article Snippet: Image-guided surgery were performed on both SKOV3/Luc and Hela/Luc xenograft models in mice ( n = 3), when the firefly luciferase activity signal obtained by the IVIS system reached about 3 × 10 8 photon/sec/cm 2 /sr. at 24 h after intraperitoneal administration of folic-AIEgen (100 μL, 40 μg/mL), malignant tissues and peritoneal tumors were identified by bioluminescent signals and AIE signals (with excitation at 430 nm) obtained by IVIS Lumina LT Series III (Perkin Elmer, USA), as well as visible fluorescence under UV lamps.

Techniques: Dissection, Injection, Fluorescence, Two Tailed Test, Staining

Journal: Nature Communications

Article Title: Aggregation-induced emission luminogens for image-guided surgery in non-human primates

doi: 10.1038/s41467-021-26417-2

Figure Lengend Snippet: a Representative process of folic-AIEgen guided SLNs dissection from rhesus macaque. b Images of the lymphatic vessels (red arrows) and SLNs (red arrowheads) under white light (i, viii) and a handheld UV lamp (ii-vii). i and ii-Before injection, iii-2 min after injection of folic-AIEgen (700 µL, 40 µg/mL) into the right areola of macaque, iv-15 min after injection, v-Separation along the lymph vessel, vi-Exposure of the SLN, vii and viii- Dissection of the SLN. c Photographs of draining LN and adjacent LN obtained from digital camera and IVIS system. d Quantitative analysis of the fluorescence intensity of draining LN and adjacent LN. e Histological analysis of the draining LN and adjacent LN. Representative images of bright field, photoluminescence of frozen section at 488 nm excitation, and H&E staining of paraffin section. Scale bars = 100 μm. The experiment was repeated at least three times.

Article Snippet: Image-guided surgery were performed on both SKOV3/Luc and Hela/Luc xenograft models in mice ( n = 3), when the firefly luciferase activity signal obtained by the IVIS system reached about 3 × 10 8 photon/sec/cm 2 /sr. at 24 h after intraperitoneal administration of folic-AIEgen (100 μL, 40 μg/mL), malignant tissues and peritoneal tumors were identified by bioluminescent signals and AIE signals (with excitation at 430 nm) obtained by IVIS Lumina LT Series III (Perkin Elmer, USA), as well as visible fluorescence under UV lamps.

Techniques: Dissection, Injection, Fluorescence, Staining, Paraffin Section

Journal: Nature Communications

Article Title: Aggregation-induced emission luminogens for image-guided surgery in non-human primates

doi: 10.1038/s41467-021-26417-2

Figure Lengend Snippet: a Representative process of folic-AIEgen guided SLNs dissection in 4T1 breast cancer-bearing mice ( n = 3). b Images of the lymphatic vessels (red arrows) and SLNs (red arrowheads) under a handheld UV lamp (i-v), and white light (vi). i-Injection, ii-3 min after subcutaneous injection of folic-AIEgen (50 µL, 40 µg/mL) around the tumor, iii-Separation along the lymph vessel, iv-Exposure of the SLN, v-Dissection of the SLN. vi. 1 and 2 represent the sentinel LNs and adjacent LN, respectively. The inner picture was obtained in bright field (top) and UV irradiation (bottom). c Images were obtained by IVIS system, and the quantitative analysis of fluorescence signals was performed between SLNs and adjacent LNs. Data are means ± SD, n = 3, Student’s two-tailed t test, ** P < 0.01. d Histological analysis of the LNs. Representative images of bright field and photoluminescence of frozen section at 488 nm excitation, and H&E staining of paraffin section. Scale bars = 100 μm. The experiment was repeated at least three times.

Article Snippet: Image-guided surgery were performed on both SKOV3/Luc and Hela/Luc xenograft models in mice ( n = 3), when the firefly luciferase activity signal obtained by the IVIS system reached about 3 × 10 8 photon/sec/cm 2 /sr. at 24 h after intraperitoneal administration of folic-AIEgen (100 μL, 40 μg/mL), malignant tissues and peritoneal tumors were identified by bioluminescent signals and AIE signals (with excitation at 430 nm) obtained by IVIS Lumina LT Series III (Perkin Elmer, USA), as well as visible fluorescence under UV lamps.

Techniques: Dissection, Injection, Irradiation, Fluorescence, Two Tailed Test, Staining, Paraffin Section

Journal: Nature Communications

Article Title: Aggregation-induced emission luminogens for image-guided surgery in non-human primates

doi: 10.1038/s41467-021-26417-2

Figure Lengend Snippet: a Time-dependent accumulation of folic-AIEgen in the SKOV3 tumors. At different time points after intraperitoneal injection of folic-AIEgen (100 µL, 40 µg/mL), mice were sacrificed and opened the abdominal cavities. A lot of tumor nodules could be seen on the surface of intestines, and a section of intestinal tract adherent with tumor nodules was randomly resected from each mouse and imaged using the digital camera, IVIS system, and microscope (bright field and fluorescence at 488 nm excitation), respectively. No obvious fluorescence signal was detected in the tumor site at 2 h postinjection. A steady increase of the fluorescence intensity in SKOV3 tumors was observed in the following 6, 12, and 24 h, and the fluorescence signal decreased at 48 h and disappeared at 72 h. Scale bars = 100 μm. b The tumor-to-normal tissue (T/N) ratio in tumor nodules with adjacent intestinal tissues at different time points after folic-AIEgen injection. Data are means ± SD, n = 3, Student’s two-tailed t test, ** P < 0.01, *** P < 0.001. c Images of the mouse peritoneum and excised tumors captured under white light (top) and 365 nm UV light (bottom). Representative H&E staining of the excised tumors. Scale bars = 1 mm. The experiment was repeated at least three times.

Article Snippet: Image-guided surgery were performed on both SKOV3/Luc and Hela/Luc xenograft models in mice ( n = 3), when the firefly luciferase activity signal obtained by the IVIS system reached about 3 × 10 8 photon/sec/cm 2 /sr. at 24 h after intraperitoneal administration of folic-AIEgen (100 μL, 40 μg/mL), malignant tissues and peritoneal tumors were identified by bioluminescent signals and AIE signals (with excitation at 430 nm) obtained by IVIS Lumina LT Series III (Perkin Elmer, USA), as well as visible fluorescence under UV lamps.

Techniques: Injection, Microscopy, Fluorescence, Two Tailed Test, Staining